For further processing into medical devices, IVD and pharmaceutical products only. Others DNase I rec GMP Grade AOF DNase I, rec., GMP Grade, AOF 102 1584029687338397 Warranty LimitationIt is the sole responsibility of the customer to determine the suitability of all raw materials, products and components in order to ensure that the final end-use product is safe for its end use; performs or functions as intended; and complies with all applicable legal and regulatory requirements. Roche makes no representations or warranties of any kind regarding the Product, express or implied, other than those expressly set forth in the terms and conditions of sale (if any), and disclaims all implied or statutory warranties including warranty of merchantability and fitness for a particular purpose. PID00000521 from bovine pancreas, recombinant, expressed in Pichia pastoris, solution 09852093103 DNase I rec., GMP Grade AOF DNase I rec., GMP Grade AOF Reagents, kits 320 mL Not Available Will be supplied as 'DNase I rec., GMP Grade AOF'. Unit of measure is "mL". 09873562001 DNase I rec., GMP Grade AOF, 200 kU DNase I rec., GMP Grade AOF, 200 kU Reagents, kits 200 kU Not Available Will be supplied as 'DNase I rec., GMP Grade AOF, 200 kU'. Unit of measure is "PC". DNase I is an essential enzyme for efficient digestion of DNA during RNA purification. en DNase I is a DNA-specific endonuclease that hydrolyzes the phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides. In mRNA in vitro synthesis applications, it helps purify the mRNA by digesting the DNA impurities in the reaction mix. In other molecular diagnostics applications, DNase I can be used to prepare genomic DNA-free RNA templates for RT-PCR. en Achieve reliable results with pure, undegraded and stable RNA. Rely on the highly purified and rigorously tested product that excludes RNase activity ensuring high yield and quality of your RNA.animal-origin-free (AOF)free of antibioticsextended testing for impuritiesmanufactured under GMPsupplied with supplementary documents for regulatory submissions. en Appearance: Colorless to slightly yellowish, solutionVolume activity (calf thymus DNA): 12-16 kU/mL (Kunitz)Identity: ConfirmedUnit Definition: One unit according to Kunitz is the amount of enzyme required to hydrolyze the calf thymus DNA at 25°C and pH 5.0 to produce an increase of 0.001/min extinction coefficient at 260 nm under the test conditions.Protein (abs. 280 nm): ≥ 1.0 mg/mLPurity (SDS-PAGE): ≥ 95 %Proteases (up to 50 U resorufin-marked casein after 17 h; 37°C): Not detectableRibonucleases (up to 10 U with MS II RNA/4 h / 37°C): Not detectableEndotoxins: < 5 EU/mLBioburden: < 5 CFU/mLHost cell protein: ≤ 100 ppmHeavy metals (Ni, Co, V): ≤ 10 ppmStability: At -15 to -25°C within specification range for 18 months. en