For further processing only. Others Proteinase K recombinant PCR Grade from Titrirachium album expressed in Pichia pastoris solution Proteinase K, rec., PCR Grade, sol. 3.6.14.8.1.6 from Titrirachium album, expressed in Pichia pastoris, solution 03654672103 Proteinase K, rec., PCR grade, solution Proteinase K, recombinant, PCR Grade 07613336132906 Reagents, kits 850 mL Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "Proteinase K, rec., PCR grade, solution". Unit of measure is "L". EC 3.4.21.64 en 39450-01-6 en Proteinase K, recombinant, PCR Grade, digests proteins very efficiently. It can be used to rapidly inactivate endogenous RNases and DNases during nucleic acid isolation or remove enzymes that have been used during (m)RNA synthesis by in vitro transcription, as in the manufacture of mRNA vaccines.Proteinase K is also used to:Isolate native RNA and DNA from tissues and cell lines by promoting cell lysisAnalyze membrane structures by modifying proteins and glycoproteins on cell surfacesRemove cellular debris during the preparation of colony liftsTreat tissue sections to ensure efficient probe infiltration during in situ hybridization en Recombinant Proteinase K in PCR Grade quality is a universal tool to remove proteins from DNA or (m)RNA preparations. en Maximize the yield of target nucleic acids.Proteinase K is rigorously tested for the absence of nucleases.Experience stability and safety.The solution is very stable and can be stored at room temperature for at least 18 months. The handling of the solution is safe and flexible. en Appearance: Clear, colorless solutionVolume activity (+37°C, hemoglobin): ≥600 U/mLSpecific activity (+37°C, hemoglobin): ≥30 U/mg proteinUnit definition (hemoglobin): One unit is the enzyme activity which releases folin positive amino acids and peptides equivalent to 1 μmol of tyrosine in 1 minute under the test conditions at 37°C.Volume activity (+25°C, Chromozym): ≥50 U/mLSpecific activity (+25°C, Chromozym): ≥2.5 U/mg proteinUnit definition (Chromozym): One unit is the enzyme activity which cleaves 18 mmol Chromozym TRY in 1 minute at +25°C.Protein (Biuret): 14.0-22.0 mg/mLUnspecific endonucleases (MWM III DNA): Not detectable in up to 200 μg after 16 hours incubation at +37°C.Nicking activity (pBR322 DNA): Not detectable in up to 200 μg after 16 hours incubation at +37°C.Ribonucleases (MS2 RNA): Not detectable in up to 40 μg after 16 hours incubation at +37°C.DNA (Threshold®): ≤10 pg/mg enzymeBioburden: ≤5 CFU/mLStability: At +2 to +8°C within specification range for 18 months. en