For further processing only. Others Pyrophosphatase recombinant GMP Grade Pyrophosphatase, rec., GMP Grade, AOF 3.1.5.4.5.2 from yeast, expressed in E. coli, solution 08140677103 Pyrophosphatase, rec., GMP Grade, 20 ml Pyrophosphatase, recombinant, GMP Grade Reagents, kits 20 mL Not Available Will be supplied as "Pyrophosphatase, rec., GMP Grade, 20 ml". Unit of measure is "piece". EC 3.6.1.1 en Use the enzyme in the synthesis of mRNA for therapeutics or vaccines. It is designed to meet specific needs of the pharmaceutical industry (fit-for-purpose):animal-origin-free (AOF)free of antibioticsextended testing for impuritiesmanufactured under GMPsupplied with supplementary documents for regulatory submissions. en Pyrophosphatase hydrolyzes inorganic pyrophosphate that is generated during in vitro transcription reactions. By removing the unwanted pyrophosphate, the reaction equilibrium shifts to the product side increasing mRNA yield. The enzyme is part of every preparative mRNA synthesis from lab-scale to industrial scale, for example in the synthesis of COVID-19 vaccines. en Pyrophosphatase improves the yield of in vitro mRNA synthesis. en Appearance: Clear, colorless to slightly yellowish solutionVolume activity: ≥ 600 U/mLUnit definition: One unit of the Pyrophosphatase catalyzes the hydrolysis of 1 µmol inorganic pyrophosphate in 1 min at 25°C.Protein (abs. 280nm): 1.8-2.2 mg/mLPurity (SDS-PAGE): ≥ 95 %Unspecific endonucleases (up to 300 ng enzyme using MWM III-DNA; 16 h/37°C): Not detectableNicking activity (up to 300 ng enzyme using pBR 322-DNA) 16 h/37°C): Not detectableRNase activity (up to 300 ng enzyme using MS II-RNA; 1 h/37°C): Not detectableProteases (50 μg/15 min/37°C): Not detectableExonucleases (using 1 μg): Not detectableEndotoxins: ≤ 10 EU/mLBioburden: ≤ 10 CFU/mLE. coli host cell DNA: ≤ 100 pg/mg ProteinE. coli host cell protein: ≤ 50 ppmHeavy metals (Ni, Co, V): ≤ 10 ppmStability: At -15 to -25°C within specification range for 36 months from date of manufacture. en